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Comparison of the effects of the dietary addition of two lactic acid bacteria on the development and conformation of sea bass larvae, Dicentrarchus labrax, and the influence on associated microbiota ArchiMer
Lamari, Faouzi; Castex, Mathieu; Larcher, Thibaut; Ledevin, Mireille; Mazurais, David; Bakhrouf, Amina; Gatesoupe, Joel.
Probiotics may have many effects on health and development of fish larvae. One of the most promising is related to spinal conformation, though the mode of action is not clearly understood. The present study attempted to investigate the effects of two strains of lactic acid bacteria on associated microbiota, histological development and gene expression. Sea bass larvae were fed since 5 dph (day post hatch) with either a standard control diet (Diet C), or the same diet supplemented with Pediococcus acidilactici MA18/5M (Diet P), or with an autochthonous strain of Lactobacillus casei (X2; Diet L). The two lactic acid bacteria were incorporated in the diets at the levels of 106 and 107 CFU (colony forming units) g-1 in two consecutive experiments,...
Tipo: Text Palavras-chave: Fish larvae; Probiotics; Microbiota; Histopathology; Gene expression; DGGE.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00108/21966/19673.pdf
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Selection of lactic acid bacteria as candidate probiotics and in vivo test on Artemia nauplii ArchiMer
Lamari, Faouzi; Sadok, Khouadja; Bakhrouf, Amina; Gatesoupe, Joel.
Lactic acid bacteria (LAB) were isolated from rotifer cultures in a marine hatchery to search for potential probiotics for marine animals. Fifteen strains were first selected among a total of 55, according to antibacterial activity against Vibrio sp. Among eight strains identified as Lactobacillus casei, four were highly adhesive, suggesting some ability for surface colonization. The other strains were identified as Lactobacillus plantarum, Lactobacillus dextrinicus, and Leuconostoc sp. To validate probiotic potential, Artemia were challenged against pathogenic Vibrio alginolyticus, with or without one of six selected LAB strains. The six strains protected Artemia against the pathogen, to some extent on condition that nutrient enrichment was provided. La....
Tipo: Text Palavras-chave: 16S rDNA; Antagonism; Artemia; Lactic acid bacteria; Vibrio.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00154/26511/24767.pdf
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Utilisation de bactéries lactiques probiotiques pour prémunir les poissons d'élevage contre des vibrions pathogènes ArchiMer
Lamari, Faouzi.
Diseases cause major production losses in fish farms. Bacteria of the genus Vibrio are known as opportunistic pathogens, and have been associated with mass-mortality episodes. To cope with these disease outbreaks, fish farmers often resort to the use of antibiotics. However, preventive overuse has led to the emergence of resistant strains and frequent treatment failures. Probiotic lactic acid bacteria are increasingly used to improve the quality of seed production in aquaculture, while limiting antibiotic treatments. Microbial strains must fulfil several criteria to be evaluated as potential probiotics.In a first step, we selected and characterized 55 strains of lactic acid bacteria isolated from fish hatchery. The first selection criterion focused on the...
Tipo: Text Palavras-chave: Probiotiques; Microflore intestinale; Réponse immunitaire; Stress oxydatif; Malformations vertébrales; Ossification; Oxidative stress; Vertebral malformations; Dicentrarchus labrax; Artemia; Vibrio; Probiotic; Intestinal microflora; Immune response.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00498/60943/64336.pdf
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